Love over Lust: Elizabeth Proctor vs. Abigail Williams Essay

What does it mean to be different? Being different means not being the same as another or just simply being unlike in form and quality. In Arthur Miller’s play The Crucible, difference is shown between the characters Abigail Williams and Elizabeth Proctor. This play portrays the historical events of the Salem witch trials through a crowd of unforgettable characters. Abigail Williams is the malicious, deceiving accuser; whereas Elizabeth Proctor is virtuous and is the wrongfully accused. To begin, Abigail Williams and Elizabeth Proctor are complete opposites of each other, one lies to save herself and avoid consequences; while the other lies to save the one she loves, John Proctor. Abigail discovers she could abstain getting in trouble by making up stories to cover up her manipulative lies and by accusing other people. She previously worked as the Proctor’s servant until Elizabeth became aware of the affair between Abigail and her husband, John. Unlike Abigail, Elizabeth Proctor does not try to create problems by lying. She is more concerned about her husband and her kids. Knowing about the affair between John and Abigail makes it more difficult for Elizabeth to forgive John. She is the faithful and religious wife of John Proctor. She lies for the very first time to save the life of her husband. At this point Elizabeth is completely unaware of the fact that her husband has confessed. Thus, Abigail is known to be the antagonist, while Elizabeth is more of a guide mentor. In The Crucible, Elizabeth Proctor is Abigail Williams foil in several ways. Abigail is a jealous, scheming and lonely; whereas Elizabeth is a religious, trustworthy and loving character. Abigail does everything she can to make it work, but then ends up running away from her problems. She is lonely and looks to John for comfort. Elizabeth is a forgiving character. She forgives her husband for all his wrong doings. John decides to keep his good name and is hanged as he chooses not to confess. Elizabeth forgives him because she truly realizes how much he loves her and how much she loves him. Overall, everyone is different and has their own way of showing it. Difference is shown through words and actions between the two characters, Abigail Williams and Elizabeth Proctor. Abigail is proven to be the misleading accuser, while Elizabeth is proven to be forgiving. Maliciousness and guilt lives in the heart of the teenage adulteress, Abigail Williams; whereas truth and innocence resides in the soul of Elizabeth Proctor.

An analysis of Who’s for the Game? By Jessie Pope Essay

War is a highly debatable topic that has influenced many poets. An issue that is important in Jessie Pope’s 1914 poem Who’s for the game? This essay will explore a range of literary devices used within the poem to help analyse the explicit and implicit meanings. Furthermore, it will use appropriate literacy terminology to back up quotes within the poem. Additionally, this essay will analyse the structure of the poem to show how meaning is conveyed. Pope, a naive poet, speaks of a highly debatable topic known to man. However, she makes reference to the war as being a game, she suggests that it is â€Å"The biggest that’s played† though, she does not anticipate the destruction which war may bring. The poem is reliant on propaganda which relied heavily on men’s guilt. It could be seen that this encouraged men to fight and defend their county. This is shown through a literary device, a metaphor. An example being, â€Å"And who wants a seat in the stand † the use of this device prominently states that spectating the war will result in deepest regret because they are not defending their country. It could be suggested that the emphasis of the metaphor helped influence men’s participation in the war. Arguably, though Pope promotes the so called glory of war, it is seen that not protecting their country could result in an invasion. Here, Pope addresses that without soldiers at war, their country could be destroyed. Alternatively, Pope makes use of personification indicating the realism of war. She writes, â€Å"Your country is up to her neck in a fight†. Here, the poet personifies their country as a woman, implying that the war is at its most extreme. This addresses the men that their country needs them the most at this point in time. In contrast, it is clear that the implicit meaning of this literary device exposes the brutality of war, vicious and scary. Jessie Pope has written her poem in a conversational manner making â€Å"Who’s for the game?† memorable and persuasive to the readers. The poet has also made use of a metrical rhythm throughout the entire stanza’s. Furthermore, the poet has made use of rhetorical questions throughout the first three stanzas. She emphasises the word â€Å"Who†, an example being, â€Å"Who’s for the game?† Pope has used the word to present the invitation of recruitment to men. It is seen that these specific questions are without a doubt persuasive, and have encouraged men of all ages to fight  in the war. Throughout the third stanza, it can be seen that Pope has replaced aggressive words with more light hearted descriptions. She speaks of â€Å"Picnic†, a known and enjoyable experience. However, Pope has used the implicit phrase in an informal manner raising the awareness that war is difficult and unpleasant. The structure of the first stanza has a three syllable foot with the first two syllables unstressed and the third one stressed. Similarly, throughout the last three stanzas the poem misses some of the unstressed syllables but the effect of the stressed syllable is predominate. The final line â€Å"And she’s looking and calling for you† draws heavily on the promotion for recruitment. The significance of â€Å"she† refers to the war as an animal being discussed. Having analysed the literary devices, terminology and structure in Who’s for the Game? It is seen that the entire poem draws heavily on men’s guilt who want to protect their country and family, although they are afraid. Pope certainly glorifies war within this poem, thought she raises false hopes for the wrong reasons. The whole poem is justification of the brutality faced when at war.

Individual Case Study Analysis Essay Example | Topics and Well Written Essays - 2000 words - 1

Individual Case Study Analysis - Essay Example The case study reflects the impact of the cultural difference between the employees of JET. The report has been prepared for understanding the key issues of the organization and focus on analysing the reasons behind these issues. It will highlight the impact of linguistic barrier and cultural differences within the organization. The different situations where the difference in the nature of thinking and the social values resulted in creation of distance between the employees of different nationality have been discussed in this study. Finally, recommendations to the organizations for solving the recent issues and improving its performance have been concluded in the report. 2. Introduction In this case study, the difference in the values of the Japanese and the Americans has been clearly shown The Japanese were formal and reserved. On the other hand, the American were informal and self assertive in nature. In case of accepting assignments in the foreign countries (as expatriates), it i s very important to consider the personality, psychology and cultural differences. ... This case study reflected the clashes which took place as a result of differences in the personality, values, psychology, culture and also lack in the orientation and training programs. It highlighted the factors which should have been considered by Kelly before planning to accept the offer. 3. Analysis and Issues Kelly found that the Japanese were too much devoted towards their work and were satisfied in working for extra time during the weekends and coming to office during the weekends. According to Kelly, the employees should make proper use of the vacation time provided to them in the job contract. Kelly noticed that none of the Japanese women have been promoted to a senior level in the Board of Education. The only women found to be working there were the young secretaries or office ladies. Mr. Higashi, who was the supervisor of the foreign participants of JET, had a tendency of arranging projects as well as other conferences and cancelling them without informing to the Assistant Language Trainers about the cancellation of such projects and conferences. In a similar manner, he arranged for special assignment during the holiday without providing prior information to the ALT’s. These were the key issues where the foreigners had to suffer because of the cultural differences. Moreover, as these foreigners could not speak the Japanese language properly, communication with them was very difficult. Moreover, the Japanese considered these foreign employees to be less committed towards their work as they never stayed in the office after the completion of the working hour at 5.00 p.m. on the weekdays and never worked during the weekends. The ALT’s had stated it clearly that there were vacation days mentioned in the job contract and they will utilise these

Marketing plan Assignment Example | Topics and Well Written Essays - 4000 words

Marketing plan - Assignment Example er and better means of connectivity to consumers and masses around the globe have increasingly provided the seeds of birth to a highly popular virtual world, thanks to the widespread popularity provided by the massive surge of online social networks and various micro blogging sites. The rise of the virtual world is strong and its presence is being actively felt by individuals around the world, as they increasingly try to gather information, work or even relax in their daily lives, irrespective of their physical presence in diverse geographical locations around the world. However, it is of utmost importance to address the fact that the access to the virtual world is only restricted by the process of accessing the World Wide Web from various highly sophisticated and latest technology based digital gadgets like Smart phones and laptops. It is of utmost relevance in this case to state that all these technological gadgets are essentially is dependent on electrical battery, which in turn acknowledges electricity as the primary source of power. Hence charging up of the electrical gadgets is highly important for the purpose of maintaining connectivity with the entire world through the access of World Wide Web (http://travellingtwo.com/resources/keeping-gadgets-charged). However, while talking in terms of charging up of the electrical gadgets, it can be increasingly said that the customers all over the world face various issues while travelling, due to the non compatibility of the charging devices with the electricity outlets. Most of them increasingly resort to the process of carrying separate connectivity devices like universal Adapter and reverse voltage charger. Hence, a new product named ‘EasyConnect’ is being introduced in the market, which will help in the process of charging of technological gadgets in the market. The most unique advantage that is being provided by the new product ‘EasyConnect’ is that it will have the dual and integrated combinations of

Post WWII Discrimination Essay Example | Topics and Well Written Essays - 750 words

Post WWII Discrimination - Essay Example All the participants of the war were entitled to government compensation due to their effort and time wasted in protecting the country. The compensations would enable the ex-soldiers improve their lives and settle fast after the mission2. The government passed Servicemen Readjustment Act in 1944 to adequately recognize the military personnel who had taken part in the war. This law would help the soldiers to settle immediately after the war and enjoy their Lives with relatives and families. However, after the war, black servicemen received unfair treatment from the state compared to their white counterparts in terms of remunerations and other individual rights envisaged in Servicemen Readjustment Acts of 1944. According to the government policies all the soldiers who had taken part in the world war two were promised inexpensive marketable and dwelling houses. All the soldiers whether back or white who had taken part in the Second World War, would have a chance to acquire the buildings of their choices on fair terms3. The government would subsidize the houses for the soldiers to enable them acquire dwelling places and settle with their families and relatives. The state had also offered to give those military men interest free loans which they could use to purchase houses at a lower than market value. However, after the end of the war white military men were given the chances to purchase the houses as promised. They were also given huge amount of loans which ere interest free to purchase the houses. On the other hand, black soldiers did not get any opportunity to buy the houses at lower than market value. They did not get any loan at all in favorable terms as promised by the government4. Consequently, the white soldiers bought good residential and commercial houses and lived a high quality life. The black soldiers on the other hand continued to live in poverty like before. This resulted to inequality in wealth possessions among the ex-soldiers in America. The gove rnment of America had promised all the soldiers who were involved in the war a chance to further their learning after the end of the war5. This offer was for both black and white soldiers, and the cost would be borne by the American government. After the war the government offered chances to white military men to pursue their studies at different education levels of their choices. The government gave the white soldiers first priority and they occupied all learning institutions6. However, most of the black ex-servicemen were denied this privilege of advancing their learning. Even those who were admitted in schools were not allowed to mix with the white soldiers. The black soldiers were given accommodation in tents because they were considered inferior and unworthy to live in the same building with the white soldiers who were considered superior7. This amounted to inequality because all soldiers had acquired similar training and fought on equal terms during the Second World War Inequa lity was also witnessed in sporting activities. Sports such as horse race, golf and swimming facilities were preserves of the white. The white soldiers enjoyed better sporting facilities while the black soldiers did not have access to gaming facilities at all. After the war the black servicemen were blamed by the white counterparts for their inability to perform excellently during the war. The white soldie

Psychology - Community service Research Paper Example | Topics and Well Written Essays - 1000 words

Psychology - Community service - Research Paper Example After a lengthy interview with the agency’s Deputy Director, I found out just how much of a toll order it is to keep these agencies running. They face numerous day to day challenges. It takes nerves of steel to keep these agencies ablaze. The Ray of Hope Agency takes care of the entire Village. It serves a population of about 4500 people. It has majorly employed people from the local area. There are however experts employed from all around the country who take care of the more sophisticated aspects. The agency has employed people from all walks of life, ranging from cleaners to cooks to mentors to psychologists and managers. Applicant employees are thoroughly vetted before being employed. Being a church based agency, Ray of Hope insists that its employees be God fearing. Employment is purely based on merit and dedication to Christianity. The agency has local agents who identify the Middle School Students who are most inclined towards dropping out of school and those who have recently dropped out and have not been subjected to the rigorous process of the agency. Once identified, the students are approached by a set of qualified psychologists who talk them into participating in the agency’s process. The students are then officially enrolled into the agency. A team of staff members is set out to investigate each student’s background and approach his or her parents or guardians. The team comes up with a report that explains the possible reasons for the students apparent drop out from school or inclination towards it. The students are then each counseled appropriately by experts. They are made to watch movies that touch on real life scenarios and the importance of education. The values of education are thoroughly highlighted and brainwashed into the student’s minds. They are kept free from demeaning peer pressure and bad habits like drug abuse and sexual encounters. They are convinced to enroll back in school. Working with their

Wk2 INTL304 Forum Coursework Example | Topics and Well Written Essays - 250 words

Wk2 INTL304 Forum - Coursework Example Human source intelligence is considered the oldest method of information collection. The intelligence is collected from human sources. Collection of such data entails clandestine acquisition of documents, photographs and other related materials1. Going for the source of information ensures that the data collected is reliable and viable. Human intelligence entails all the information that is directly obtained from various human sources. It includes a wide range of activities from direct observation and reconnaissance to the use of spies and informants. The source of information is of essence since the information can be distorted when being moved from one source to the other. It is important to evaluate the target of collection before actual collection is done to avoid confusion and to ensure the information is thoroughly collected2. The intelligent information may end up not being viable in cases where wrong targets are selected. It may also take a long time to reach the source if the wrong targets are the ones selected in the beginning. Being aware of the source of intelligence information will help to identify the magnitude of the threat and thereby coming up with effective mitigation measures. Intelligence officials are t herefore tasked with a mandate of ensuring that the information collected is reliable and of

On time plz Essay Example | Topics and Well Written Essays - 1000 words

On time plz - Essay Example Supervisors and managers are under the scope of responsibilities of HR professionals insofar as overseeing the accomplishment of their respective tasks in view of the overall accomplishment of organizational goals. A job is a group of positions having similar or related duties and responsibilities. The tasks and responsibilities of each group are distinctly different from those of other groups. (Martires 446) Before hiring workers, available jobs have to be classified and corresponding job information described mainly in terms of duties and responsibilities. The concept of culture in organization is critical since it influences the philosophy and practices of management which works through people. It is vital that managers understand the people’s values, beliefs, needs and motivation. Managing corporate culture is a skill which every top officer should learn since culture change is a complex and long process involving coordinated efforts by top leadership which is very visible to their subordinates. Executives must change their behavior and through role modeling send signals to their constituents. If the culture is not what the organization members want, it can be turned around to fit the mission and vision, strategy, structure, resources and technology of the organization. The well run corporations have developed cultures that are distinctive and responsible for creation, implementation and maintenance of their world leadership positions. In this regard, all phases of operations, strategy and staffing, and organizational culture have to be considered by human resources for the efficient and effective accomplishment of organizational goals. Part 4: You are the HR staffing specialist at a very successful 25 year old company that manufactures home entertainment products: HDTVs, home-theatre equipment, etc. The manager of the marketing division has just left

Justice right and the state Essay Example | Topics and Well Written Essays - 750 words

Justice right and the state - Essay Example Rawls underlines that in violating this basic right a person has failed to do a fundamental duty. Minimax theory means a rule which can be applied to all decisions in order to determine the maximum possible loss. In contrast, maximin theory implies rules which can help a decision-maker to increase the minimum gain. Rawls states that both prniples can be seen as a reasonable conception of justice. He argues that: There is an analogy between the two principles and the maximin rule for choice under uncertainty. . . . The maximin rule tells us to rank alternatives by their worst possible outcomes: we are to adopt the alternative the worst outcome of which is superior to the worst outcome of the others' (Rawls 1971, pp.152-3). For instance, the conditions stipulate that contracting parties follow 'the maximin rule'. Then, they will strive to maximise the supreme welfare level of the least advantaged. The Difference Principle developed by Rawls suggests that it is fundamentally concerned not with absolutes but with relativities (Freeman, 2002). 'The maximin rule' does not demand, as the Difference Principle does, that people must never allow any advance above "the benchmark of equality', save in so far as this advance is 'to the advantage of the least fortunate" (Ralws 2005, p. 153). Still, according to the maximin rule every person can advance but in case others are not deprived their rights. It is important to state that 'disadvantaging' has to be understood as making worse off, not as making worse off just comparatively, and without any modification of further conditions (Freeman, 2002). This discussion leads researchers to one of the things about both the application to maximin in Rawls and the Difference Principle. Rawls wants both of the rulers to be applied always, and without inquiry into the level of the minimum. In real life situation, those solid, ordinary, and not irrational customers suggest by the relative diffidence of the pools element in their regular budgets that up to some acceptable minimum standard of living they maximin; and then, but only then, maximax. Rawls is thinking of all social goods as distributed by some authority (Pogge and Kosch, 2007). Such a distribution, of what is all at bottom property, can only be a zero sum function: if one individual lacks something, then the reason is solely that it has been allocated to someone else. So what an outsider might see as one individual becoming better off at no one else's expense, looks from inside the world of 'justice as fairness' like that individual being needlessly given what might have been i ssued instead to another, and ought to have been (Daniels, 1989). The entire argument in Rawls states that all the goods of every kind which have been, are, or will be produced or discovered within their to them unknown nationwide territory, are now available, free of any prior claims, for distribution at the fair collective judgment of the contractors. The various good or ill deserts of the other several characters must all be grounded upon accidents and contingencies; that is, the contingent facts about what they did or failed to do. All such particular and essentially claims about right and desert are in the broadest sense moral and as such disputatious ("Political Egalitarianism" 2008). This discussion allows me to say that Rawls follows maximin principles in his theory of Justice. He states: "[social

Operations Management as an integral component of a manufacturing Essay

Operations Management as an integral component of a manufacturing industry - Essay Example MRP ensures that there is just about enough materials available for production. If this planning is not efficiently done, there is the likelihood of either finding oneself in a situation where the materials for production is less than required leading to disruption in production or having too much of materials resulting in wasteful spending. MRP has evolved with the computer technology. Its full potential embracing all aspects is prevalent in data base software and there is tremendous scope for utilization of all the heads and sub-heads for the purpose of manufacture of unique and desirable product. It operates on the basis of a master schedule and develops the requirement for materials. The software is programmed to procure different materials for a product. MRP has to be tuned to production planning. Otherwise there will be mismatch in procurement. Normally, MRP through the use of computer is good for mass production where the volume of manufacture is high. Production planning is a part of the master schedule and sees through the MRP. It is an input to capacity plan. The MRP software is complex and so the user has to be provided training so that he is in a position to apply his knowledge in the use the software to the actual requirement of the organization. He must be capable of understanding the software to maximize its use so that the computer is not seen as something that only produces reports with information of non-relevance and disuse. Once the details in the customers' orders are fed into the integrated database, the system checks the schedule as against the current capacity. If the inputs of the materials requirement exceed current capacity, it provides the alternatives for increasing capacity or creating adjustments in the master schedule. The process of MRP must suit its environment to optimize production. In other words, business adapts MRP to suit the market. The computerized version of MRP is complex and is designed to cater to high volumes (Material Requirement Planning MRP). While on the subject, it is necessary to gauge the break-even point with MRP. There are several ways of analyzing the break-even point. Costs and sales forecast analysis is one of them (Decision Making). Total Quality Management (TQM) Total Quality Management is that aspect of management where there is awareness of maintaining quality at every level. According to the International Organization for Standardization, "TQM is a management approach for an organization, centered

The rate of catalase action Essay Example for Free

The rate of catalase action Essay I have to wear goggles, prevent any accidents that can damage my eyes I have to make sure I handle all the glassware carefully I have to handle the hydrogen peroxide very carefully because it is very toxic Method 1. Set up the experiment according to the diagram 2. Measure 10 cm of catalase (celery juice) 3. Measure 10 cm of Hydrogen Peroxide 4. Pour the catalase into the conical flask 5. Fill the burette with water 6. Pour the hydrogen peroxide into the conical flask and close the bung and start the timer 7. Record the volume of gas given off every 10 seconds () 8. Mix hydrogen peroxide with pure water to give the following percentage of concentration of acid: 100%, 80%, 60%, 40% and 20% 9. Repeat 1 8 using the 100%, 80%, 60%, 40% and 20% hydrogen peroxide 10. Repeat the whole experiment three times to get a more reliable result 11. Plot graphs for each concentration -volume of gas collected against time 12. Draw best-fit lines Diagram Analysis After I have done the experiment, I have got the results: 1st result Time (Seconds) Volume of gas collected when there is 100% of H O (cm ). Volume of gas collected when there is 80% of H O (cm ) Volume of gas collected when there is 20% of H O (cm ) 1. 5 2. 6 3. 7 5. 0 6. 2 7. 5 8. 6 9. 6 Now I am going to draw the graphs volume of gas collected against time. The graphs shows a curve best-fit line that means whenever the concentration increases, the volume of gas that was collected in the given time increases but the volume of gas collected increase at a slower rate at the end. The 100% concentration produced the most oxygen in the shortest time, which gives it a higher reaction rate than the others. According to the graph, it has proven my prediction is correct. The highest concentration would produce the most oxygen in the shortest time. The volume of gas collected increases at a slower rate at the end because the towards the end, there are less substrate and enzyme left, so it takes longer to react and releases the gas(oxygen). The rate of reaction is a measure of how fast the reaction takes place. A reaction will take place when the particles of the reacting substances collide with each other and a fixed amount of energy called the activation energy is reached. If a collision between particles can produce sufficient energy (i. e. if they collide fast enough and in the right direction) a reaction will take place. This is called the Collision Theory for rates of reaction. Concentration of the substrate increases, the greater the rate of reaction will be because higher concentration means a higher number of particles in a given volume. If there are more particles, there will be more reaction. More reaction means a larger number of successful collisions. Therefore the catalase action rate will increase. Also, the particles are closer together, so they can collide more often. The more often they collide, the chance of reaction happening increases. So the catalase action rate increases. Evaluation I think the results are very good that I can use them to support what I predicted at the beginning. I also have enough evidence to support my conclusion. The points on my graph are not very close to the best-fit line which is due to the large scale I used, so the gap seems to be bigger. But the points let me draw reasonable best-fit lines and they also show the pattern I expect. I think I can still rely on the results because they show the general pattern for each concentration. I know my results are reliable because they fitted my prediction and it also enables me to explain the relation between concentration and rate of catalase reaction. Also, I repeated my experiment three times to obtain the results, so I know they are reliable. The appropriate apparatus I used, increases the accuracy of the results. I think my experiment is quite successful as I can collect results that help me to draw some accurate graphs except there are a few anomalous points. I think this happened because: 1) I did not close the bung quick enough and some gas leak out which cause a decrease in the readings 2) I did not use the same celery each time because 1 celery does not provide sufficient catalase for the whole experiment which can cause increase or decrease to the readings 3) The temperature is slightly different as I did the experiment in three different days which can cause increase or decrease to the readings I think there is a better way of doing it that is to have a tap at the top. When I open it, the hydrogen peroxide will drop down into the catalase and I do not need to put the bung on because the bung is put on top of the tap which means no gas will leak out. Also, when I time it with a stopwatch, it will not be as accurate as using computers to time because human has reaction time that will increase the time taken and this will make the experiment less accurate. I can do the experiment in this way: For this experiment, I have used celery as a source of catalase, other than this; I can use potatoes or liver that may affect the time taken to collect gas, as they are different. Further experiment Planning Apparatus A 50cm conical flask with a bung A burette Clamps and stand Delivery tube Container 10cm measuring cylinder A stopwatch. 20 vol of hydrogen peroxide Potato tubes (catalase) Safety control I have to make sure all the school bags and chairs are under the table, in case I trip over I have to wear goggles, prevent any accidents that can damage my eyes I have to make sure I handle all the glassware carefully I have to handle the hydrogen peroxide very carefully because it is very toxic Method 1. Set up the experiment according to the diagram 2. Measure 10 g of catalase (potato juice) 3. Measure 10 cm of Hydrogen Peroxide 4. Pour the catalase into the conical flask 5. Fill the burette with water 6.Set up the light sensor and the computer 7. Turn on the tap and start the computer at the same time 8. Record the volume of gas given off every 10 seconds (10s, 20s, 30s, 40s, 50s, 60s, 70s and 80s) 9. Mix hydrogen peroxide with pure water to give the following percentage of concentration of acid: 100%, 80%, 60%, 40% and 20% 10. Repeat 1 9 using the 100%, 80%, 60%, 40% and 20% hydrogen peroxide 11. Repeat the whole experiment three times to get a more accurate result 12. Plot graphs for different concentration- volume of gas collected against time 13. Draw best-fit lines 14.

Augmented Reality Ar Is A Live Media Essay

Augmented Reality Ar Is A Live Media Essay Hardware components for augmented reality are: processor, display, sensors and input devices. Modern mobile computing devices like smartphones and tablet computers contain these elements which often include a camera and MEMS sensors such as accelerometer, GPS, and solid state compass, making them suitable AR platforms.[8] [edit] Display Various technologies are used in Augmented Reality rendering including optical projection systems, monitors, hand held devices, and display systems worn on ones person. [edit] Head-mounted A head-mounted display (HMD) is a display device paired to a headset such as a harness or helmet. HMDs place images of both the physical world and virtual objects over the users field of view. Modern HMDs often employ sensors for six degrees of freedom monitoring that allow the system to align virtual information to the physical world and adjust accordingly with the users head movements.[9][10][11] HMDs can provide users immersive, mobile and collaborative AR experiences.[12] [edit] Eyeglasses AR displays can be rendered on devices resembling eyeglasses. Versions include eye wear that employ cameras to intercept the real world view and re-display its augmented view through the eye pieces[13] and devices in which the AR imagery is projected through or reflected off the surfaces of the eye wear lens pieces.[14][15][16] [edit] Contact lenses Contact lenses that display AR imaging are in development. These bionic contact lenses might contain the elements for display embedded into the lens including integrated circuitry, LEDs and an antenna for wireless communication.[17][18][19][20] Another version of contact lenses, in development for the U.S. Military, is designed to function with AR spectacles, allowing soldiers to focus on close-to-the-eye AR images on the spectacles and distant real world objects at the same time.[21][22] [edit] Virtual retinal display A virtual retinal display (VRD) is a personal display device under development at the University of Washingtons Human Interface Technology Laboratory. With this technology, a display is scanned directly onto the retina of a viewers eye. The viewer sees what appears to be a conventional display floating in space in front of them.[23] [edit] EyeTap The EyeTap (also known as Generation-2 Glass[24]) captures rays of light that would otherwise pass through the center of a lens of an eye of the wearer, and substituted each ray of light for synthetic computer-controlled light. The Generation-4 Glass[24] (Laser EyeTap) is similar to the VRD (i.e. it uses a computer controlled laser light source) except that it also has infinite depth of focus and causes the eye itself to, in effect, function as both a camera and a display, by way of exact alignment with the eye, and resynthesis (in laser light) of rays of light entering the eye.[25] [edit] Handheld Handheld displays employ a small display that fits in a users hand. All handheld AR solutions to date opt for video see-through. Initially handheld AR employed fiduciary markers,[26] and later GPS units and MEMS sensors such as digital compasses and six degrees of freedomaccelerometer-gyroscope. Today SLAM markerless trackers such as PTAM are starting to come into use. Handheld display AR promises to be the first commercial success for AR technologies. The two main advantages of handheld AR is the portable nature of handheld devices and ubiquitous nature of camera phones. The disadvantages are the physical constraints of the user having to hold the handheld device out in front of them at all times as well as distorting effect of classically wide-angled mobile phone cameras when compared to the real world as viewed through the eye.[27] [edit] Spatial Spatial Augmented Reality (SAR) augments real world objects and scenes without the use of special displays such as monitors, head mounted displays or hand-held devices. SAR makes use of digital projectors to display graphical information onto physical objects. The key difference in SAR is that the display is separated from the users of the system. Because the displays are not associated with each user, SAR scales naturally up to groups of users, thus allowing for collocated collaboration between users. SAR has several advantages over traditional head-mounted displays and handheld devices. The user is not required to carry equipment or wear the display over their eyes. This makes spatial AR a good candidate for collaborative work, as the users can see each others faces. A system can be used by multiple people at the same time without each having to wear a head-mounted display. Examples include shader lamps, mobile projectors, virtual tables, and smart projectors. Shader lamps mimic and augment reality by projecting imagery onto neutral objects, providing the opportunity to enhance the objects appearance with materials of a simple unit- a projector, camera, and sensor. Handheld projectors further this goal by enabling cluster configurations of environment sensing, reducing the need for additional peripheral sensing.[28][29] Other tangible applications include table and wall projections. One such innovation, the Extended Virtual Table, separates the virtual from the real by including beam-splitter mirrors attached to the ceiling at an adjustable angle.[30] Virtual showcases, which employ beam-splitter mirrors together with multiple graphics displays, provide an interactive means of simultaneously engaging with the virtual and the real.[31][32] Altogether, current augmented reality display technology can be applied to improve design and visualization, or function as scientific simulations and tools for education or entertainment. Many more implementations and configurations make spatial augmented reality display an increasingly attractive interactive alternative.[11] Spatial AR does not suffer from the limited display resolution of current head-mounted displays and portable devices. A projector based display system can simply incorporate more projectors to expand the display area. Where portable devices have a small window into the world for drawing, a SAR system can display on any number of surfaces of an indoor setting at once. The drawbacks, however, are that SAR systems of projectors do not work so well in sunlight and also require a surface on which to project the computer-generated graphics. Augmentations cannot simply hang in the air as they do with handheld and HMD-based AR. The tangible nature of SAR, though, makes this an ideal technology to support design, as SAR supports both a graphical visualisation and passive haptic sensation for the end users. People are able to touch physical objects, and it is this process that provides the passive haptic sensation.[7][33][34][35] [edit] Tracking Modern mobile augmented reality systems use one or more of the following tracking technologies: digital cameras and/or other optical sensors, accelerometers, GPS, gyroscopes, solid state compasses, RFID and wireless sensors. These technologies offer varying levels of accuracy and precision. Most important is the position and orientation of the users head. Tracking the users hand(s) or a handheld input device can provide a 6DOF interaction technique.[36] [edit] Input devices Techniques include speech recognition systems that translate a users spoken words into computer instructions and gesture recognition systems that can interpret a users body movements by visual detection or from sensors embedded in a peripheral device such as a wand, stylus, pointer, glove or other body wear.[37][38][39][40] [edit] Computer The computer analyzes the sensed visual and other data to synthesize and position augmentations. [edit] Software and algorithms A key measure of AR systems is how realistically they integrate augmentations with the real world. The software must derive real world coordinates, independent from the camera, from camera images. That process is called image registration which uses different methods of computer vision, mostly related to video tracking.[41][42] Many computer vision methods of augmented reality are inherited from visual odometry. Usually those methods consist of two parts. First detect interest points, or fiduciary markers, or optical flow in the camera images. First stage can use feature detection methods like corner detection, blob detection, edge detection or thresholding and/or other image processing methods.[43][44] The second stage restores a real world coordinate system from the data obtained in the first stage. Some methods assume objects with known geometry (or fiduciary markers) present in the scene. In some of those cases the scene 3D structure should be precalculated beforehand. If part of the scene is unknown simultaneous localization and mapping (SLAM) can map relative positions. If no information about scene geometry is available, structure from motion methods like bundle adjustment are used. Mathematical methods used in the second stage include projective (epipolar) geometry, geometric algebra, rotation representation with exponential map, kalman and particle filters, nonlinear optimization, robust statistics. [edit] Applications Augmented reality has many applications, and many areas can benefit from the usage of AR technology. AR was initially used for military, industrial, and medical applications, but was soon applied to commercial and entertainment areas as well.[45] [edit] Archaeology AR can be used to aid archaeological research, by augmenting archaeological features onto the modern landscape, enabling archaeologists to formulate conclusions about site placement and configuration.[46] Another application given to AR in this field is the possibility for users to rebuild ruins, buildings, or even landscapes as they formerly existed.[47] [edit] Architecture AR can aid in visualizing building projects. Computer-generated images of a structure can be superimposed into a real life local view of a property before the physical building is constructed there. AR can also be employed within an architects work space, rendering into their view animated 3D visualizations of their 2D drawings. Architecture sight-seeing can be enhanced with AR applications allowing users viewing a buildings exterior to virtually see through its walls, viewing its interior objects and layout.[48][49] [edit] Art AR technology has helped disabled individuals create art by using eye tracking to translate a users eye movements into drawings on a screen.[50] An item such as a commemorative coin can be designed so that when scanned by an AR-enabled device it displays additional objects and layers of information that were not visible in a real world view of it.[51][52] [edit] Commerce ViewAR BUTLERS App Placing furniture using AR AR can enhance product previews such as allowing a customer to view whats inside a products packaging without opening it.[53] AR can also be used as an aid in selecting products from a catalog or through a kiosk. Scanned images of products can activate views of additional content such as customization options and additional images of the product in its use.[54][55] AR is used to integrate print and video marketing. Printed marketing material can be designed with certain trigger images that, when scanned by an AR enabled device using image recognition, activate a video version of the promotional material.[56][57][58] [edit] Education Augmented reality applications can complement a standard curriculum. Text, graphics, video and audio can be superimposed into a students real time environment. Textbooks, flashcards and other educational reading material can contain embedded markers that, when scanned by an AR device, produce supplementary information to the student rendered in a multimedia format.[59][60][61] Students can participate interactively with computer generated simulations of historical events, exploring and learning details of each significant area of the event site.[62] AR can aide students in understanding chemistry by allowing them to visualize the spatial structure of a molecule and interact with a virtual model of it that appears, in a camera image, positioned at a marker held in their hand.[63] Augmented reality technology also permits learning via remote collaboration, in which students and instructors not at the same physical location can share a common virtual learning environment populated by vi rtual objects and learning materials and interact with another within that setting.[64] [edit] Everyday 30 years of Augmediated Reality in everyday life. Since the 1970s and early 1980s, Steve Mann has been developing technologies meant for everyday use i.e. horizontal across all applications rather than a specific vertical market. Examples include Manns EyeTap Digital Eye Glass, a general-purpose seeing aid that does dynamic-range management (HDR vision) and overlays, underlays, simultaneous augmentation and diminishment (e.g. diminishing the electric arc while looking at a welding torch).[65] [edit] Industrial design AR can help industrial designers experience a products design and operation before completion. Volkswagen uses AR for comparing calculated and actual crash test imagery.[66] AR can be used to visualize and modify a car body structure and engine layout. AR can also be used to compare digital mock-ups with physical mock-ups for efficiently finding discrepancies between them.[67][68] [edit] Medical Augmented Reality can provide the surgeon with information, which are otherwise hidden, such as showing the heartbeat rate, the blood pressure, the state of the patients organ, etc. In particular AR can be used to let the doctor look inside the patient by combining one source of images such as an X-ray with another such as video. This helps the doctor to identify the problem with the patient in a more intuitive way than looking at only type of image data. This approach works in a similar as the technicians doing maintenance work. Examples include a virtual X-ray view based on prior tomography or on real time images from ultrasound and confocal microscopy probes[69] or visualizing the position of a tumor in the video of an endoscope.[70] AR can enhance viewing a fetus inside a mothers womb.[71] See also Mixed reality. [edit] Military In combat, AR can serve as a networked communication system that renders useful battlefield data onto a soldiers goggles in real time. From the soldiers viewpoint, people and various objects can be marked with special indicators to warn of potential dangers. Virtual maps and 360Â ° view camera imaging can also be rendered to aid a soldiers navigation and battlefield perspective, and this can be transmitted to military leaders at a remote command center.[72] [edit] Navigation Augmented reality map on iPhone AR can augment the effectiveness of navigation devices. Information can be displayed on an automobiles windshield indicating destination directions and meter, weather, terrain, road conditions and traffic information as well as alerts to potential hazards in their path.[73][74][75] Aboard maritime vessels, AR can allow bridge watch-standers to continuously monitor important information such as a ships heading and speed while moving throughout the bridge or performing other tasks.[76] [edit] Office workplace AR can help facilitate collaboration among distributed team members in a work force via conferences with real and virtual participants. AR tasks can include brainstorming and discussion meetings utilizing common visualization via touch screen tables, interactive digital whiteboards, shared design spaces, and distributed control rooms.[77][78][79] [edit] Sports and entertainment AR has become common in sports telecasting. Sports and entertainment venues are provided with see-through and overlay augmentation through tracked camera feeds for enhanced viewing by the audience. Examples include the yellow first down line seen in television broadcasts of American football games showing the line the offensive team must cross to receive a first down. AR is also used in association with football and other sporting events to show commercial advertisements overlaid onto the view of the playing area. Sections of rugby fields and cricket pitches also display sponsored images. Swimming telecasts often add a line across the lanes to indicate the position of the current record holder as a race proceeds to allow viewers to compare the current race to the best performance. Other examples include hockey puck tracking and annotations of racing car performance and snooker ball trajectories. [41][80] AR can enhance concert and theater performances. For example, artists can allow listeners to augment their listening experience by adding their performance to that of other bands/groups of users.[81][82][83] The gaming industry has benefited a lot from the development of this technology. A number of games have been developed for prepared indoor environments. Early AR games also include AR air hockey, collaborative combat against virtual enemies, and an AR-enhanced pool games. A significant number of games incorporate AR in them and the introduction of the smartphone has made a bigger impact.[84][85] [edit] Task support Complex tasks such as assembly, maintenance, and surgery can be simplified by inserting additional information into the field of view. For example, labels can be displayed on parts of a system to clarify operating instructions for a mechanic who is performing maintenance on the system.[86][87] Assembly lines gain many benefits from the usage of AR. In addition to Boeing, BMW and Volkswagen are known for incorporating this technology in their assembly line to improve their manufacturing and assembly processes.[88][89][90] Big machines are difficult to maintain because of the multiple layers or structures they have. With the use of AR the workers can complete their job in a much easier way because AR permits them to look through the machine as if it was with x-ray, pointing them to the problem right away.[91] [edit] Tourism and sightseeing Augmented reality applications can enhance a users experience when traveling by providing real time informational displays regarding a location and its features, including comments made by previous visitors of the site. AR applications allow tourists to experience simulations of historical events, places and objects by rendering them into their current view of a landscape.[92][93][94] AR applications can also present location information by audio, announcing features of interest at a particular site as they become visible to the user.[95][96] [edit] Translation AR systems can interpret foreign text on signs and menus and, in a users augmented view, re-display the text in the users language. Spoken words of a foreign language can be translated and displayed in a users view as printed subtitles How Augmented Reality Works Video games have been entertaining us for nearly 30 years, ever since Pong was introduced to arcades in the early 1970s. Computer graphics have become much more sophisticated since then, and game graphics are pushing the barriers of photorealism. Now, researchers and engineers are pulling graphics out of your television screen or computer display and integrating them into real-world environments. This new technology, called augmented reality, blurs the line between whats real and whats computer-generated by enhancing what we see, hear, feel and smell. On the spectrum between virtual reality, which creates immersive, computer-generated environments, and the real world, augmented reality is closer to the real world. Augmented reality adds graphics, sounds, haptic feedback and smell to the natural world as it exists. Both video games and cell phones are driving the development of augmented reality. Everyone from tourists, to soldiers, to someone looking for the closest subway stop can now benefit from the ability to place computer-generated graphics in their field of vision. Augmented reality is changing the way we view the world or at least the way its users see the world. Picture yourself walking or driving down the street. With augmented-reality displays, which will eventually look much like a normal pair of glasses, informative graphics will appear in your field of view, and audio will coincide with whatever you see. These enhancements will be refreshed continually to reflect the movements of your head. Similar devices and applications already exist, particularly on smartphones like the iPhone. In this article, well take a look at where augmented reality is now and where it may be headed soon. Augmenting Our World The basic idea of augmented reality is to superimpose graphics, audio and other sensory enhancements over a real-world environment in real time. Sounds pretty simple. Besides, havent television networks been doing that with graphics for decades? However, augmented reality is more advanced than any technology youve seen in television broadcasts, although some new TV effects come close, such as RACEf/x and the super-imposed first down line on televised U.S. football games, both created by Sportvision. But these systems display graphics for only one point of view. Next-generation augmented-reality systems will display graphics for each viewers perspective. Some of the most exciting augmented-reality work is taking place in research labs at universities around the world. In February 2009, at the TED conference, Pattie Maes and Pranav Mistry presented their augmented-reality system, which they developed as part of MIT Media Labs Fluid Interfaces Group. They call it SixthSense, and it relies on some basic components that are found in many augmented reality systems: Camera Small projector Smartphone Mirror These components are strung together in a lanyardlike apparatus that the user wears around his neck. The user also wears four colored caps on the fingers, and these caps are used to manipulate the images that the projector emits. SixthSense is remarkable because it uses these simple, off-the-shelf components that cost around $350. It is also notable because the projector essentially turns any surface into an interactive screen. Essentially, the device works by using the camera and mirror to examine the surrounding world, feeding that image to the phone (which processes the image, gathers GPS coordinates and pulls data from the Internet), and then projecting information from the projector onto the surface in front of the user, whether its a wrist, a wall, or even a person. Because the user is wearing the camera on his chest, SixthSense will augment whatever he looks at; for example, if he picks up a can of soup in a grocery store, SixthSense can find and project onto the soup information about its ingredients, price, nutritional value even customer reviews. By using his capped fingers Pattie Maes says even fingers with different colors of nail polish would work a user can perform actions on the projected information, which are then picked up by the camera and processed by the phone. If he wants to know more about that can of soup than is projected on it, he can use his fingers to interact with the projected image and learn about, say, competing brands. SixthSense can also recognize complex gestures draw a circle on your wrist and SixthSense projects a watch with the current time. Mistry demonstrates SixthSense Photo courtesy Sam Ogden, Pranav Mistry, MIT Media Lab The SixthSense augmented reality system lets you project a phone pad onto your hand and phone a friend without removing the phone from your pocket. See more gadget pictures. Photo courtesy Lynn Barry, Pranav Mistry, MIT Media Lab Augmented Reality on Cell Phones While it may be some time before you buy a device like SixthSense, more primitive versions of augmented reality are already here on some cell phones, particularly in applications for the iPhone and phones with the Android operating system. In the Netherlands, cell phone owners can download an application called Layar that uses the phones camera and GPS capabilities to gather information about the surrounding area. Layar then shows information about restaurants or other sites in the area, overlaying this information on the phones screen. You can even point the phone at a building, and Layar will tell you if any companies in that building are hiring, or it might be able to find photos of the building on Flickr or to locate its history on Wikipedia. Layar isnt the only application of its type. In August 2009, some iPhone users were surprised to find an augmented-reality easter egg hidden within the Yelp application. Yelp is known for its user reviews of restaurants and other businesses, but its hidden augmented-reality component, called Monocle, takes things one step further. Just start up the Yelp app, shake your iPhone 3GS three times and Monocle activates. Using your phones GPS and compass, Monocle will display information about local restaurants, including ratings and reviews, on your cell phone screen. You can touch one of the listings to find out more about a particular restaurant. There are other augmented reality apps out there for the iPhone and other similar phones and many more in development. Urbanspoon has much of the same functionality as Yelps Monocle. Then theres Wikitude, which finds information from Wikipedia about sites in the area. Underlying most of these applications are a phones GPS and compass; by knowing where you are, these applications can make sure to offer information relevant to you. Were still not quite at the stage of full-on image recognition, but trust us, people are working on it. Weve looked at some of the existing forms of augmented reality. On the next page, well examine some of the other applications of the technology, such as in video games and military hardware An iPhone user displays the augmented reality app Monocle, which combines the phones camera view with tiny tags indicating the names, distances and user ratings of nearby bars, restaurants and more. AP Photo/Marcio Jose Sanchez Augmented Reality in Video Games and the Military Video game companies are quickly hopping aboard the augmented-reality locomotive. A company called Total Immersion makes software that applies augmented reality to baseball cards. Simply go online, download the Total Immersion software and then hold up your baseball card to a webcam. The software recognizes the card (and the player on it) and then displays related video on your computer screen. Move the card in your hands make sure to keep it in view of the camera and the 3-D figure on your screen will perform actions, such as throwing a ball at a target. Total Immersions efforts are just the beginning. In the next couple of years, well see games that take augmented reality out into the streets. Consider a scavenger-hunt game that uses virtual objects. You could use your phone to place tokens around town, and participants would then use their phones (or augmented-reality enabled goggles) to find these invisible objects. Demos of many games of this order already exist. Theres a human Pac-Man game that allows users to chase after each other in real life while wearing goggles that make them look like characters in Pac-Man. Arcane Technologies, a Canadian company, has sold augmented-reality devices to the U.S. military. The company produces a head-mounted display the sort of device that was supposed to bring us virtual reality that superimposes information on your world. Consider a squad of soldiers in Afghanistan, performing reconnaissance on an opposition hideout. An AR-enabled head-mounted display could overlay blueprints or a view from a satellite or overheard drone directly onto the soldiers field of vision. Now that weve established some of the many current and burgeoning uses of augmented reality, lets take a look at the technologys limitations and what the future holds. Augmented reality can breathe a little life into your sports trading cards. Photo courtesy Total Immersion Limitations and the Future of Augmented Reality Augmented reality still has some challenges to overcome. For example, GPS is only accurate to within 30 feet (9 meters) and doesnt work as well indoors, although improved image recognition technology may be able to help [source: Metz]. People may not want to rely on their cell phones, which have small screens on which to superimpose information. For that reason, wearable devices like SixthSense or augmented-reality capable contact lenses and glasses will provide users with more convenient, expansive views of the world around them. Screen real estate will no longer be an issue. In the near future, you may be able to play a real-time strategy game on your computer, or you can invite a friend over, put on your AR glasses, and play on the tabletop in front of you. There is such a thing as too much information. Just as the CrackBerry phenomenon and Internet addiction are concerns, an overreliance on augmented reality could mean that people are missing out on whats right in front of them. Some people may prefer to use their AR iPhone applications rather than an experienced tour guide, even though a tour guide may be able to offer a level of interaction, an experience and a personal touch unavailable in a computer program. And there are times when a real plaque on a building is preferable to a virtual one, which would be accessible only by people with certain technologies. There are also privacy concerns. Image-recognition software coupled with AR will, quite soon, allow us to point our phones at people, even strangers, and instantly see information from their Facebook, Twitter, Amazon, LinkedIn or other online profiles. With most of these services people willingly put information about themselves online, but it may be an unwelcome shock to meet someone, only to have him instantly know so much about your life and background. Despite these concerns, imagine the possibilities: you may learn things about the city youve lived in for years just by pointing your AR-enabled phone at a nearby park or building. If you work in construction, you can save on materials by using virtual markers to designate where a beam should go or which structural support to inspect. Paleontologists working in shifts to assemble a dinosaur skeleton could leave virtual notes to team members on the bones themselves, artists could produce virtual graffiti and doctors could overlay a digital image of a patients X-rays onto a mannequin for added realism. The future of augmented reality is clearly bright, even as it already has found its way into our cell phones and video game systems. For more information about the subject and where its headed, take a look at the links on the next page Augmented Reality Augmented reality (AR) is a live, direct or indirect, view of a physical, real-world environment whose elements are augmented by computer-gener

Monitoring Therapeutic Drugs: Strategies

Monitoring Therapeutic Drugs: Strategies This article provides an introduction into some of the current techniques and assays utilised in Therapeutic Drug Monitoring (TDM) TDM is a multi disciplinary function that measures specific drugs at intervals to ensure a constant therapeutic concentration in a patient blood stream. The selection of an analytical technique for TDM involves a choice between immunoassay and chromatography technique. Once the methodology has been chosen, there are also numerous options available within these categories including FPIA, EMIT, KIMS, HPLC and nephelometric immunoassay. An overview of each method is given and its processing of drugs. The future outlook in the methodology involved in TDM is also explored and discussed. INTRODUCTION Therapeutic drug monitoring (TDM) is a multi disciplinary function that measures specific drugs at selected intervals to ensure a constant therapeutic concentration in a patient blood stream. (Ju-Seop Kang Min Hoe Lee) The response to most drug concentrations is therapeutic, sub-therapeutic or toxic and the main objective of TDM is to optimize the response so the serum drug concentration is retained within the therapeutic range. When the clinical effect can be easily measured such as heart rate or blood pressure, adjusting the dose according to the response is adequate (D.J. Birkett et al). The practice of TDM is required if the drug meets the following criteria: Has a narrow therapeutic range If the level of drug in the plasma is directly proportional to the adverse toxic If there is appropriate applications and systems available for the management of therapeutic drugs. If the drug effect cannot be assessed by clinically observing the patient (Suthakaran and C.Adithan) A list of commonly monitored drugs is given in table 1. The advances in TDM have been assisted by the availability of immunoassay and chromatographic methods linked to detection methods. Both techniques meet the systemic requirements of sensitivity, precision and accuracy. Within both methods are many numerous options and will be further explored in this title. Ideally the analytical method chosen should distinguish between drug molecules and substances of similar composition, detect minute quantities, be easy to adapt within the laboratory and be unaffected by other drugs administrated. An overview of the current analytical techniques and future trends in TDM is emphasised in this title and its role in laboratory medicine. NEPHLEOMETRIC IMMUNOASSAY AND its USE IN TDM Immunoassays play a critical role in the monitoring of therapeutic drugs and a range of techniques in which the immunoassay can be existed exist. Nephleometric immunoassays are widely used for TDM and are based on the principle of hapten drug inhibition of immunoprecipitation. The precipitation is measured using nephelometric principles that measure the degree of light scattering produced. In some cases Turbidmetry principles can be applied to measure precipitation via the amount of transverse light. In nephleometric immunoassays, if the drug molecule is a monovalent antigenic substance, a soluble immunocomplex is formed. However if the drug molecule is a multivalent antigenic substance, whereby two drug moieties are conjugated to a carrier protein, the conjugate reacts with the antibody to form an insoluble complex. The insoluble complex may compose of numerous antigens and antibodies, thus scattering the light. Therefore nephleometry of turbidmetry techniques are required to measure the reaction. In respect to this principle precipitation inhibition of a drug can be measured. The test sample (serum) is introduced to a fixed quantity of polyhaptenic antigen and anti drug antibody. The serum drug antigen competes with polyhaptenic antigen for binding to the anti drug antibody. Any free drug present in the sample inhibits the precipitation between the antibody and polyhaptenic antigen. Therefore the drug concentration ids indirectly proportional to the formation of precipitate whi ch is quantified by a nephelometer. The more polyhaptenic antigen present, the more precipitate is formed until the maximum is encountered. Further addition of antigen causes a reduction in the amount of precipitate formed due to antigen excess. The use of nephelometric immunoassay for TDM is termed competitive due to the competitive binding for the sites on the antibody by the antigen. It also distinguishes the drug assay system from the conventional nephleometric immunoassay for proteins. Variations of this assay exist including: The use of saliva or CSF may be used as an alternative to serum. Both alternative matrixes contain less light scattering molecules and so a larger volume of sample is used in order to compensate. Turbidmetric methods may also be applied to quantitative immunoprecipation . turbidmetric analysis is preformed at a lower wavelength and similarly detects immunoprecipation like nephelometric techniques. End point analysis of immunoprecipitaion is commonly employed, however rate analysis is also applicable. Addition of formaldehyde blocks further precipitation and is utilised in end point analysis. Agglutination inhibition immunoassay can also be detected by nephelometric immunoassay systems in which the drug or hapten is directly linked onto the surface of the particle and is generally suitable for low serum drug concentration while precipitation inhibition detects concentration above 1ug/ml If homologus and heterologus drug concentrations are utilized for antibody and polyhaptenic antigen preparations, sensitivity and specificity may be increased. Polyclonal and monoclonal antibodies may be employed in this assay. The use of monoclonal antibodies removes any interference caused by antibody cross reactivity. Choosing a hybrid cell with the most desirable antibody is difficult and therefore is most likely to be less sensitive than the use of polyclonal antibodies Overall the nephelometric immunoassay is an excellent assay system for TDM. Advantages over other assay systems include its simplicity, speed and low cost. It is a homogenous method that requires no separation steps or isotopes. Only two reagents are required in limited amounts as if the antibody to antigen ratio is not optimum, the sensitivity is decreased. This is due to the formation of less precipitate in the absence of drug. In the presence of a drug, inhibition is less efficient. The sensitivity of the assay depends on antibody hapten binding, however it yields high specificity. Therefore nephelometric precipitation inhibition immunoassays are a novel technique in the clinical practice of TDM. (Takaski Nishikawa Vol 1, 1984) FLUORESCENCE POLARIZATION IMMUNOASSAY AND its USE IN TDM Fluorescence polarization immunoassay(FPIA) is a widely used 2 step homogenous assay that is conducted in the solution phase and is based on a rise in fluorescence polarization due to the binding of the fluorescent labelled antigen with antibody. The first step of the immunoassay involves the incubation of the serum sample with none labelled anti drug antibody. If the patient sample contains drug molecules, immune complexes will form between antibody and antigen. The second stage of this assay involves the addition of a flourscein labelled antigen (tracer) into the mixture(.Jacqueline Stanley 2002) The purpose of the flourscein tracer is to bind on any available sites on the drug specific antibody for detection purposes. If the first stage occurred in which the anti drug antibody formed a complex with the drug from the sample, less or no antigen binding sites will be available for the tracer to bind to. Consequently a higher proportion of the flourscein tracer is unbound in the solut ion. If the sample contains no drug an antigen, Step 1 does not occur and the anti drug antibodies will bind the flourscein antigen tracer. In this assay the degree of polarization is indirectly proportional to the concentration of drug present. (: Chris Maragos 2009) Fluorescence polarization is calculated to determine the concentration of drug present. Fluorscein labelled molecules rotate when they interact with polarised light. Larger complexes rotate less then smaller complexes and therefore remain in the light path. When the large immune complex is labelled with a fluorescent tracer, it is easily detected once present in the light path. If no drug was present in the sample, the availability of binding sites on the antibody entices the fluorscein tracer to bind, restricting its motion resulting in a higher degree of polarisation, Thus it is easy to identify that polarization is indirectly proportional to the concentration of drug present. The benefit of utilising FPIA in TDM includes the elimination of processed to separate bound and free labels, an indicator that this assay is time efficient. An unique feature of this assay is that the label used is a flurophore and the analytical signal involves the measurement of the fluorescent polarizatio n. ( Jacqueline Stanley 2002) A standard curve is constructed to determine the concentration of drug present and is easily reproducible due to the stability of the reagents utilized and the simplicity of the method. However FPIA has some limitations and is prone to interference from light scattering and endogenous fluorescent tracers in the samples. To help overcome these limitations variations on the technique is employed including: Use of a long wavelength label The fluorscein tracers utilized produce adequate signals, however light scattering events can interfere with these signals. The use of a long wavelength label permits extended fluorescence relaxation times which may be more sensitive for the detection of high molecular weight antigens on drugs. Use of CE-LIF The use of capillary electrophoresis with laser induced fluorescence detection enhances the sensitivity of this method. This competitive FPIA separates free and antibody bound tracers and utilizes LIFP as a detection system.( David S. Smith Sergei A 2008) Overall FPIA has proven to be a time and cost effective, accurate and sensitive technique in TDM and remains one of the most promising methods in this clinical field. ENZYME MULTIPLIED IMMUNOASSAY TECHNIQUE AND its USE IN TDM Enzyme Multiplied Immunoassay Technique (EMIT) is an advanced version of the general immunoassay technique utilising an enzyme as a marker. EMIT is a 2 stage assay that qualitatively detects the presence of drugs in urines and quantitatively detects the presence of drugs in serum.( David S. Smith Sergei A )Both the competitive and non-competitive forms of this assay are homogenous binding based that rapidly analyze microgram quantities of drug in a sample. in the competitive assay, the patient sample is incubated with anti drug antibodies. Antibody antigen reactions occur if there is any drug present in the sample. The number of unbound sites indirectly correlates with the drug concentration present. The second step involves the addition of an enzyme labelled specific drug which will bind to available binding sites on the antibody inactivating the enzyme. A enzyme widely used in EMIT assays is Glucose 6 Phosphate Dehydrogenase which primarily oxidises the substrate added (Glucose 6 Phosphate). The co-factor NAD+ is also reduced to NADH by the active enzyme. Any enzyme drug conjugate that is unbound remains active, therefore only in this case , can the oxidation of NAD+ to NADH occur. An increase in absorbance photometrically @ 340nm correlates with the amount of NADH produced. (Jacqueline Stanley 2002) A non competitive format of this assay also exists, where by drug specific antibodies are added in excess to the sample resulting in antigen antibody interactions if the drug is present. A fixed amount of enzyme drug conjugate is then added which occupy any unbound sites present on the antibody. The active enzyme that is unbound oxidised NAD+ to NADH indicating presence of free enzyme conjugate and subsequently drug molecules present. (chemistry.hull.ac.uk/) EMIT technology is becoming increasingly popular as a method to monitor therapeutic drug levels. Drugs monitored using this technique includes anti asthmatic drugs, anti epileptic drugs and cardio active drugs. Radioimmunoassay work on the same principle as competitive EMIT with the exception of the use of a radio isotope as a marker. Gamma radiation is emitted from the marker leading to a high level of sensitivity and specificity. As it uses radio isotopes it is not the most cost effective in todays modern environment. MICROPARTICLE IMMUNOASSAY AND its USE IN TDM Microparticle agglutination technology uses latex microparticles and plays a leading role in TDM in the quantitative measurement of carbarbapenzaine, phenytoin, theophylline and phenybarbital. Kinetic movement of microparticles in solution (KIMS) is a homogenous assay and is based on the principle of competitive binding between microparticles via covalent coupling. When free drug exists in the patient sample, it will bind to the antibody present. As a result the microparticle antigen complex fail to bind with the antibody and the formation of a particle aggregate does not occur. Micro particles in solution fail to scatter light causing a low absorbance reading. If the patient sample is negative for the drug, the micro particle drug complex binds to the antibodies. The complex that is formed upon binding blocks the transmitted light and causes light scattering resulting in increasing absorbance readings. Hence the degree of light scattering is inversely related to the concentration of drug present. Light scattering spectroscopy improves the sensitivity and quantitation of particle based immunoassays, thus making KIMS a highly sensitive and accurate technique in TDM. Its popularity has developed throughout the years for many reasons. Reagents required for this assay are in expensive and have high stability. KIMS is a universal assay and can be preformed on a variety of analyzers. The assay has minimal interference as a change of absorbance is measured as a function of time while absorbance readings of interfering substances do not alter with time.( Frederick P. Smith, Sotiris A. Athanaselis) CHROMATOGRAPHY AND its USE IN TDM For many years liquid chromatography has been linked to detection systems and its application in TDM is becoming incredibility popular. Liquid chromatography was initially employed in response to difficulties arising in Gas Chromatography (G.C) due to heat instability and non specific adsorption on surfaces. High Performance Liquid chromatography is the main chromatography technique utilized for TDM. Thin Layer Chromatography (T.L.C) and Gas Chromatography are other alternatives, however have limitations that suppress their use in TDM. A derivatization step must be performed for highly polar and thermo liable drugs for G.C to be successful. TLC has a poor detection limit and is unable to detect low concentration of drug present. HPLC has revolutionized the monitoring of TDM with rapid speed and sensitivity of analysis and can separate a wider variety of drugs compared to GC and TLC. For this reason, HPLC is considered the most widely adaptable chromatographic technique when coupled w ith UV detection and Mass Spectrophotometry for TDM.( Phyllis R. Brown, Eli Grushka) BASIC PRINCIPLES IN HPLC HPLC is a separation technique performed in the mobile phase in which a sample is broken down into its basic constituents. HPLC is a separation technique that employs distribution differences of a compound over a stationary and mobile phase. The stationary phase is composed of a thin layer created on the surface of fine particles and the mobile phase flows over the fine particles while carrying the sample. Each component in the analyse moves through the column at a different speed depending on solubility in the phases and on the molecule size. As a result the sample components move at different paces over the stationary phase becoming separated from one another. Drugs that are localised in the mobile phase migrate faster as to those that are situated in the stationary phase. The drug molecules are eluted off the column by gradient elution. Gradient elution refers to the steady change of the eluent composition and strength over the run of the column. As the drug molecules elute of HPL C is linked to a detection system to detect the quantity of drug present in the sample. Detection systems include mass spectrophotometry and UV detection. (Mahmoud A. Alabdalla Journal of Clinical Forensic Medicine) DETECTION SYSTEMS USED IN HPLC FOR TDM Detection of HPLC with a diode array ultraviolet detector has proved to be a sustainable application system in the identification after HPLC analysis. The use of UV detection allows the online possession the compounds UV spectra. These detection system absorb light in the range of 180-350nm. UV light transmitted passes through a sensor and from that to the photoelectric cell. This output is modified to appear on the potentiometric recorder. By placing a monochromatoer between and light source and the cell, a specific wavelength is created for the detection , thus improving the detectors specificity. A wide band light source can also be used as an alternative method. In this case the light from the cell is optically dispersed and allowed to fall on the diode array.( Mahmoud A. Alabdalla Journal of Clinical Forensic Medicine) HPLC can also be coupled to a mass spectrophotometer as a detection method. Mass spectrophotometry (MS) elucidates the chemical structure of a drug. Sensitivity of this technique is observed as it can detect low drug concentration in a sample. Specificity of this method can be futher enhanced by Tandem mass spectrophotometric analysis. This involves multiple steps of mass spectrophotometry. This is accomplished by separating individual mass spectrometer elements in space or by separating MS phases in time. (Franck Saint-Marcoux et al) FUTURE TRENDS IN TDM METHODOLOGY AGILENTS 1200 HPLC MICRO CHIP Agilents 1200 HPLC micro chip technology combines microfliudics with an easy use interface that confines the HPLC procedure tot his dynamic chip. The micro chip technology integrates analytical columns, micro cuvette connections and a metal coated electro spray tip into the chip to function as a regular HPLC analyzer. The compact chip reduces peak dispersion for a complete sensitive and precise technique. The microchip comes complete with an integrated LC system into sample enrichment and separation column. The operation of the chip is well defined and manageable upon insertion into the Agilent interface which mounts onto the mass spectrophotometer. The built in auto sampler loads the samples and the sample is moves into the trapping column by the mobile phase. Gradient flow from the pump moves the sample from the trapped column to the separation column. The drug is separated the same as the convention methods however reduced peak dispersion does produce better separation efficiency than the conventional method. This form of technology is currently in use in the United States but has not developed outside of the U.S(http://www.agilent.com) PHYZIOTYPE SYSTEM This is the latest application on the market for the treatment and monitoring of drugs associated with metabolic disorders. The PhyzioType system utilizes DNA markers from several genes coupled with biostatisical knowledge to predict a patients risk of developing adverse drug reactions. (Kristen k. Reynolds Roland Valdes) AMPLICHIP CYP450 TEST The Amplichip CYP450 Test is a new technology that has revolutionised the TDM of anti psychotic drugs. This test has been approved by the FDA in 2006 but is not currently in use in laboratories in Ireland. This test is used for the analysis of CYP2D6 and CYP2C19 genes, both of which have an influence in drug metabolism. The function of this test is to identify a patient genotype so their phenotype is calculated. Based on the patient phenotype, a clinician determines the type of therapeutic strategy he/she will commence (Kristen k. Reynolds Roland Valdes) DISCUSSION This paper illustrates the increasing role of immunoassay and chromatography techniques in the clinical laboratory routine monitoring of therapeutic drugs. Before an analytical technique is introduced into TDM it must meet the requirements of sensitivity, accuracy and specificity needed for most TDM applications. The methodology of TDM in todays clinical setting revolves around the use of immunoassays and chromatography techniques. A range of immunoassays was discussed revolving around their principle and advantages and limitations. The majority of immunoassays utilised in the TDM are homogenous based for rapid analysis and efficient turn around time for drug monitoring. Most immunoassays involved in TDM are based on the same principle of competitive binding for antibody. The factor that distinguishes each immunoassay is the detection methods used. Detection methods discussed in this reviewed include nephelometric techniques, flourscein labels, enzyme labels and the use of micro part icles. Each method relies on different detection principles as discussed, however characteristics common to all methods include accuracy, sensitivity and specificity. The methodologies discussed also are time and cost efficient, both essential in laboratory assays. Chromatographic techniques are also discussed with HPLC providing the most impact to TDM. Gas and thin layer chromatography are other chromatographic techniques, however neither can be utilised in TDM due to the limitations both techniques hold against TDM. . HPLC is a rapid sensitive method for the quantitation of drugs in a sample and for this reason is the most widely adaptable chromatographic technique applied in TDM. Like all chromatographic techniques drugs are separated based on the interaction of the drug with the stationary phase which determines the elution time. Detection methods primarily used are UV detection and mass spectrophotometry The final thought on this overview of TDM was an insight into the future of its methodology and applications .Future and approved methods are discussed given a brief outline on each. The constant development of methodologies and techniques in this area of TDM are ongoing constantly keeping the area of TDM one of the most fastest and interesting in clinical medicine. Literature Review: The Impact Of Legalized Abortion Literature Review: The Impact Of Legalized Abortion The publication of the controversial paper on legalised abortion and its affect on the rate of crime by Levitt and Donohue (2001) has resulted in widespread condemnation from a variety of sources, for example, Joseph Scheidler, executive direction of the Pro-Life Action league, described the paper as so fraught with stupidity that I hardly know where to start refuting it Crime fell sharply in the United States in the 1990s, in all categories of crime and all parts of the nation. Homicide rates plunged 43 percent from the peak in 1991 to 2001, reaching the lowest levels in 35 years. The Federal Bureau of Investigations (FBI) violent and property crime indexes fell 34 and 29 percent, respectively, over that same period. (Levitt, 2004) In his journal The impact of Legalized abortion on crime Levitt attempts to offer evidence that the legalization of abortion in 1973 was the chief contributor to the recent crime reductions of the 1990s. Levitts hypothesis is that legalized abortion may lead to reduced crime either through reductions in cohort sizes or through lower per capita offending rates for affected cohorts. The smaller cohort that results from abortion legalization means that when that cohort reaches the late teens and twenties, there will be fewer young males in their peak crime years, and thus less crime. He argues that the decision in Roe v Wade constitutes an abrupt legal development that can possibly have an abrupt influence 15-20 years later when the cohorts born in the wake of liberalized abortion would start reaching their peak crime years. In essence, Levitt puts forward the theory that unwanted children are more likely to become troubled adolescents, prone to crime and drug use, than wanted children are. As abortion is legalized, a whole generation of unwanted births are averted leading to a drop in crime two decades later when this phantom generation would have grown up. To back up this point, Levit t makes use of a platform from previous work such as (Levine et al 1996) and (Comanor and Phillips 1999) who suggest that women who have abortions are those most likely to give birth to children who would engage in criminal activity. He also builds on earlier work from (Loeber and Stouthamer-Loeber 1986) who concludes that an adverse family environment is strongly linked to future criminality. Although keen not to be encroach into the moral and ethical implications of abortion, Levitt, through mainly empirical evidence is able to back up his hypothesis by concluding that a negative relationship between abortion and crime does in fact exist, showing that an increase of 100 abortions per 1000 live births reduces a cohorts crime by roughly 10 per cent and states in his conclusion that legalized abortion is a primary explanation for the large drops in crime seen in the 1990s. One of the criticisms that can be levied against this study is its failure to take into consideration the effect other factors may have had in influencing crime rates during the 1980s and 1990s, such as the crack wave. Accounting for this factor, the abortion effect may have been mitigated slightly. Also Levitts empirical work failed to take into account the greater number of abortions by African Americans who he distinguishes as the race which commit the most amount of violent crime, and his evidence fails to identify whether the drop in crime was due to there being a relative drop in the number of African Americans. The list of possible explanations for the sudden and sharp decrease in crime during the 1990s doesnt stop at Levitts abortion/crime theory and Levitt himself in his 2004 paper identifies three other factors that have played a critical role. The first is the rising prison population that was seen over the same time period, and (Kuziemko and Levitt, 2003) attribute this to a sharp rise in incarceration for drug related offences, increased parole revocation and longer sentences handed out for those convicted of crimes, although there is the possibility of a substitution effect taking place where punishment increases for one crime, potential criminals may choose to commit alternative crimes instead. There are two ways that increasing the number of person incarcerated could have an influence on crime rates. Physically removing offenders from the community will mean the avoidance of any future crime they may plausibly commit during the time they are in prison known as the incapacitation affect. Also there is the deterrence effect, through raising the expected punishment cost potential criminals will be less inclined to commit a crime. As criminals face bounded rationality, expected utility gained from crime will have an effect on the amount of time spent devoted to crime. (Becker, 1968). A study conducted by Spelman (2000) examined the affect the incarceration rate would have on the rate of crime and finds the relationship to have an elasticity measure of -0.4 which means that an increase in the levels of incarceration of one percent will lead to a drop in crime of 0.4%. In Economic models of crime such as Becker (1968), improvements in the legitimate labor market make crime less attractive as the return earned from legitimate work increases. Using this model, the sustained economic growth that was seen in the 1990s (Real GDP per capita grew by almost 30% between 1991 and 2001 and unemployment over the same period fell from 6.8 to 4.8 percent) could be seen as a contributing factor to the drop in crime witnessed and many scholars (such as) have come to that conclusion. However, the improved macroeconomic performance of the 90s is more likely to be relevant in terms of crimes that have financial gains such as burglary and auto theft and does not explain the sharp decrease seen in homicide rates. Also, the large increase in crime seen in the 1960s coincided with a decade of improving economic growth, further corroborating the weak link between macroeconomics and crime (Levitt, 2004). One other explanation for the drop in crime and the most commonly cited reason can be seen in the growing use of police innovation, and an adoption of community policing. The idea stemmed from the broken window theory, which argues that minor nuisances, if left unchecked, turn into major nuisances (Freakonomics) The main problem with the policing explanation is that innovative police practices had been implemented after the crime rate had already began declining, and perhaps more importantly, the rate of crime dropped in cities that had not experienced any major changes in policing (Ouimet, 2004).

Imagery And Symbolism in William Blake’s The Tyger Essay -- William Bl

Imagery And Symbolism in William Blake’s The Tyger â€Å"Can you give to the horse mightyness? Can you clothe its neck with a rustling mane? Can you cause it to leap like a locust?†(Job 39:19-20) William Blake’s The Tyger is reminiscent of when God questioned Job rhetorically about his creations, many of them being fearsome beasts such as the leviathan or the behemoth. Much like this speech from the old testament, The Tyger also uses a significant amount of imagery and symbolism which contributes to its spiritual aspects. There is a wealth of imagery in the first two lines alone. The poem begins: â€Å"Tyger! Tyger! burning bright In the forests of the night,† The reader conceives in their mind the image of a tiger with a coat blazing like fire in the bowels of a dark forest. This creates a negative impression of the tiger, so some might say that the tiger is symbolic of evil. Some people may go even further to conclude that the tiger is a symbol of Satan. Perhaps mainly the people who derive their interpretation of hell from Dante’s Inferno, or other works of literature that portray the devil as a predator, cloaked in flames residing in the darkness of hell. The same type of imagery and symbolism is used in the first two lines of the second stanza, where it says: â€Å"In what distant deeps or skies Burnt the fire of thine eyes?† The images of â€Å"distant deeps or skies† again presents images of a realm of darkness, and one is reminded again of the traditional interpretation o...

Ultimately Disgusting :: essays research papers

Ultimate fighting is one of the most brutal and barbaric sports ever to be seen on television. Ultimate fighting is a no-rules fighting competition, designed to provide violent entertainment for its viewers. In this competition a fighter is allowed to punch, kick, and choke his opponent to win a fight. Ultimate fighting first came to the United States in 1993 when SEG began promoting it as, 'The Ultimate Fighting Championship.'; Ultimate fighting is an unsafe sport and people should not be permitted to view or compete in such violent competitions. Dr. George Lindberg editor of the Journal of the American Medical Association says, 'Someone's spinal cord could be fractured, an arm could be broken, and choking could result in brain damage. The possibility of injury is very high'; (Sokolove 1). The American Medical Association wants an all out ban on boxing and ultimate fighting. Senator John McCain, the leader in the opposition wants it banned because he is concerned about injury to the competitors (Kirby 20). In the UFC competitors have received broken arms, damaged their spinal cord, and been knocked out on several occasions. In a recent event in the Ukraine, a competitor died after he was beated badly. After the fight he collapsed, was taken to a hospital, and later died (Kodi). Cockfighting is banned in almost all states in this country, however in most states ultimate fighting still goes unregulated. Why are we allowing humans to compete in events that we have decided is too dangerous for animals (Sokolove 1)? Isn't allowing this type of event to go on telling our children that it is ok to fight? This type of event serves no positive purpose, and only increases the tolerance for violence in our society. Fans and promoters of ultimate fighting argue that fighters should be allowed to compete in any type of violent event if both participants are consenting. Isn't there a point then where all of this goes to far though? Suppose someone wants to televise the death of someone who consents to being killed. Under their logic this type of thing would be ok because everyone is consenting. We have already banned such consenting activities such as prostitution, drugs, and assisted suicide; we should just add this to the list. John McCain believes that the fighter's consent is deceptive. He says a fighter is, 'driven by profits or the enticements of publicity associated with it and unknowingly is placing his or her life at risk (Kirby 20).

Child Labor Essay -- Essays Papers

Child Labor Child labor has been around for long time and it still exists in today's world. Thiskind of labor provides problems or difficulties in the economic world. Child labor is social problem with the rise of industrial production and capitalism. It appeared in earlier ages in agricultural societies when the children all around the world had to work along with 19th century, spreading to many countries. The problems started when many children, younger than ten years old, were employed by factories. In this paper I will address the issues and history of child labor, the child labor laws, and my suggestions or solutions to child labor problems. Child labor is defined as the employment of children under the age of physical maturity for long hours. Child labor is often used in societies where industries are being developed. The governments and international organization usually consider a person economically active if the person works on a regular basis. A child is a laborer if the child is economically active. The children are used because they are agile, efficient at many unskilled and semi skilled work, quick learners, unprotected and easy for adults to exploit. Historically, children working is nothing new, it was and still is often described for a family's survival. Many of these children are forced into the workforce to become beggars, farm hands and factory workers. These children are denied an education and normal childhood, some children are confined and beaten. Some are denied freedom of movement or the right to leave the workplace and go to their families. Some are abducted and forced to work. Human rights abuses in these practices are existing. There are approximately 250 milli... ...ause criminal is always going to be a criminal. My opinion is that there should be a trade restriction because I want to see some changes in these countries thatuse child labor and trade restrictions on them would be my best choice. Bibliography: 1. â€Å"Child Labor†, http://1rights.igc.org/projects/childlabor/ 2. â€Å"History Of Child Labor†, http://www.atchison.K12.ks.us/gifted/childlabor.html 3. UNICEF, â€Å"Child Protection†, http://www.unicef.org/protection/index_childlabour.html 4. Human Rights Watch, â€Å"Child Labor†, http://www.hrw.org/children/labor.html 5. â€Å"Child Labor Laws†, http://www.doli.state.mn.us/childlbr.html 6. â€Å"US Child Labor Law†, http://www.cofc.edu/~muellerr/childlabor.html 7. â€Å"Children In Factories†, http://www.globalmarch.org/factory-lab/indonesia.html 8. â€Å"Kaushik Basu†,